Forms for CFG Services

Confidential
Center for Functional Genomics
Transgenic Mouse Facility · Room B210

Mouse and ES Cell Clone Screening Form

Dot Blot or Southern Blot Analysis

To be able to detect the transgenic founders by Dot blot or Southern blot, we will require 1 mg of purified probe in a concentration of 0.1 mg/ml. Please follow Instructions for DNA Cleaning and Shipping. The probe should be at least 300 bp in length. This is required for efficient labeling of the random priming reaction. In addition we would like the investigator's assurance that the probe does not cross hybridize with endogenous sequences (the investigator should run tests to proof this, see below). Also, we will need the hybridization and washing conditions to be used. If the investigator would require a Southern blot to detect the transgenic founders then a map should be included indicating the expected restriction enzymes to be used for digestion and the expected fragment sizes.

* Indicates Required Fields

The Investigator should supply a picture of the gel and autoradiograph with the following controls:

1. 5 mg of mouse genomic DNA
2. 5 mg of mouse genomic DNA + 1 copy of DNA construct
3. 5 mg of mouse genomic DNA + 5 copies of DNA construct
4. 5 mg of mouse genomic DNA + 10 copies of DNA construct
5. 5 copies of DNA construct
6. Molecular weight marker